Remerciez-le!

Remerciez @Admin pour avoir partagé cet document gratuitement, de la manière la plus simple, en partageant sur les réseaux sociaux.

Elisa technical manual and troubleshooting guide

ELISA Technical Manual and Troubleshooting Guide v CContents Kit Storage Guidelines Materials and Equipment Required Performing the ELISA Assay Sample Handling and Storage Troubleshooting Guide Page - - CBioChek ELISA Test Kit Storage Guidelines ? General ? Storage of a complete kit ? Using the kit ? Handling of partially used plates General The kit is composed of coated microtiter plates sample diluent substrate bu ?er stop solution and positive and negative controls aqueous solutions containing salts conjugate reagent aqueous solution with antibodies and enzymes washbu ?er and freeze dried substrate tablets The most critical components of the kit are the conjugate and microtiter plates These are coated at precise concentrations with antigens that are immobilized to the surface The coated microtiter plates are sensitive to moisture and temperature Moisture will a ?ect the stability of the binding of the antigen to the microtiter plates therefore it is important to keep the kit box away from water vapour or ice High temperatures C F or low temperatures C F may have a similar e ?ect The other components are due to the packing materials only sensitive to temperature The complete kit is most stable at a temperature between ?? C F Storage of a complete kit The kit should be stored at ?? C F Often there is condensation on the walls of the refrigerator therefore make sure the kit doesn ? t come in contact with the walls of the refrigerator Also prior to storing the kit or components of a kit in the refrigerator make sure there is no condensation on the kit components Using the kit When planning to run an assay remove from the refrigerator only the number of microtiter plate s that will be needed along with all the kit components and controls at least hours prior to starting the assay Handling of partially used plates In some instances only a ? partial plate ? may be required When working with partial plates cover the unused wells of the plate with adhesive tape i e scotch prior to and during the assay After the assay dry mark and cover the used wells with adhesive tape Then REMOVE the tape from the unused wells to avoid condensation and place the used plate with desiccant pouch in a resealable bag Specify the date and number of remaining wells Put the plate back in the original kit box and in the refrigerator CMaterials and Equipment Required for Running a BioChek ELISA Test ? Room with temperature between and C - F ? Refrigerator to store kits ? Freezer to store samples ? Personal computer IBM compatible with minimum GB RAM with BioChek software ? Precision Pipette and disposable tips which are able to pipette ?l i e single channel adjustable - l ? or channel pipettes repeater pipettes with disposable tips able to pipette volumes of and ?l i e - l and - l ? well at bottom dilution plates min vol well - l ? Distilled