Transwell® Permeable Supports Selection and Use Guide Table of Contents Introdu

Transwell® Permeable Supports Selection and Use Guide Table of Contents Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Selecting the Right Transwell Membrane and Pore Size. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Selecting the Right Transwell Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Using Transwell Permeable Supports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 General Directions for Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Helpful Hints. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Polyester (PET) Transwell Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Collagen-Coated Transwell-COL Inserts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Netwell™ Inserts and Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Polycarbonate Membrane Transwell Inserts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Snapwell™ Inserts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 HTS Transwell-24 Well Permeable Supports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6, 12, and 24 Well Cell Culture Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 HTS Transwell-96 Well Permeable Supports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2 Introduction Cell and tissue culture techniques are becoming increasingly important for basic and applied life science research. The development of new culture vessels and cell attach­ ment substrates is currently being driven by the need to produce an environment that resembles the in vivo state as closely as possible to enable the growth of specialized cell types. Consequently, using permeable supports with microporous membranes have become a ­ standard method for culturing these cells. These permeable supports have allowed signifi­ cant improvements in culturing polarized cells since these permeable supports permit cells to uptake and secrete molecules on both their basal and ­ apical surfaces and thereby carry out metabolic activities in a more natural fashion. Membrane filters have been used as cell growth substrates since the transfilter metaneph­ ric induction studies of Grobstein (Nature, 172:860-872; 1953). Adapted over the years to a var­ iety of cell types and numerous applications, permeable supports treated for cell growth are now recognized as providing significant advantages over solid, impermeable cell growth ­ substrates. For epithelial and other cell types, the use of permeable supports in vitro allows cells to be grown and ­ studied in a polarized state under more natural con­ ditions. Cellular ­ differentiation can also proceed to higher levels resulting in cells that mor­ phologically and functionally better represent their in vitro counterparts. Cellular functions such as transport, absorption and secretion can also be studied since cells grown on permeable supports provide convenient, independent access to apical and basolateral plasma membrane domains. The use of permeable support systems for cell cul­ ture has proven to be an invaluable tool in the cell biology laboratory. Features Transwell® cell culture inserts are convenient, sterile, easy-to-use permeable support devices for the study of both anchorage-dependent and anchorage-independent cell lines. w Designed to produce a cell culture environment that closely resembles the in vivo state w  Allows polarized cells to feed basolaterally and thereby carry out metabolic activities in a more ­ natural fashion w  Unique self-centered hanging design prevents medium wicking between the insert and outer well w Permits access to the lower compartment through windows in the insert wall w Suspended design allows for undamaged co-culturing of cells in the lower compartment w  Available in a range of pore sizes and different membranes to satisfy diverse experimen­ tal ­ requirements Selecting the Right Transwell Membrane and Pore Size Transwell permeable supports are available in three membrane materials: polycarbonate (PC), polyester (PET), and ­ collagen-coated polytetrafluoroethylene (PTFE). See Table 1 for ­ additional information on these membrane characteristics. SEM of the surface of a 0.4 µm pore polycarbonate membrane SEM of a PTFE membrane showing its pore structure The polyester Transwell-Clear inserts in a 6 well plate show the clarity of the membrane These 12 mm diameter Transwell inserts have a polycarbonate membrane Table 1. Characteristics of Transwell Membranes Polyester Polycarbonate Collagen-coated Characteristics (PET) (PC) (PTFE) Optical properties Clear Translucent Clear when wet Cell visibility Good Poor Cell outlines Tissue culture treated Yes Yes No Membrane thickness 10 µm 10 µm 50 µm Matrix/ECM coatable Yes Yes Yes Collagen treated No No Yes Available Pore Sizes (µm) 0.4, 1.0, 3.0, 8.0 0.4, 3.0, 5.0, 8.0 0.4, 3.0 3 w Polyester Transwell®-Clear inserts feature a microscopically transparent polyester uploads/Societe et culture/ transwell-guide.pdf

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• Publié le Mai 09, 2022
• Catégorie Society and Cultur...
• Langue French
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