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Kit anti toxo igg NovaLisa TM Toxoplasma gondii IgG Avidity Test Enzyme immunoassay for the avidity determination of IgG-class antibodies to Toxoplasma gondii in human serum Enzymimmunoassay zur Bestimmung der Avidit? t von IgG-Antik? rpern gegen Toxoplas

NovaLisa TM Toxoplasma gondii IgG Avidity Test Enzyme immunoassay for the avidity determination of IgG-class antibodies to Toxoplasma gondii in human serum Enzymimmunoassay zur Bestimmung der Avidit? t von IgG-Antik? rpern gegen Toxoplasma gondii in Humanserum Only for in-vitro diagnostic use English Deutsch Francais Italiano Espanol For further languages please contact our authorized distributors Bibliography Literatur Bibliographie Bibliogra ?a Bibliografía Symbols Key Symbolschlüssel Explication des symboles Legenda Símbolos Summary of Test Procedure Kurzanleitung Testdurchführung Résumé de la procedure de test Schema della procedura Resumen de la técnica Page Seite Page da Pagina Página Page Seite Page Pagina Página Page Seite Page Pagina Página Page Seite Page Pagina Página to bis à a a Product number TOXGA Determinations CENGLISH INTRODUCTION The avidity determination is a diagnostic method which is used to di ?erentiate a recent acute and a more distant past infection with Toxoplasma gondii in patient sera Avidity is the binding force of the antibody serum specimen with the corresponding antigen Low avid IgG antibodies in the early stage of infection can be di ?erentiated from high avid antibodies associated with a past infection The determination of IgG antibody avidity is an additional analysis to the classic serology in regard to the status of a Toxoplasma gondii infection INTENDED USE The NovaTec Toxoplasma gondii IgG avidity test is intended to di ?erentiate between past and acute infection PRINCIPLE OF THE ASSAY Microtiter strip wells coated with Toxoplasma antigen are incubated with diluted serum specimen dual pipetting After washing one well is incubated with avidity reagent and the corresponding well with washing bu ?er In this step the low avidity antibodies are removed from the antigens whereas the high avidity ones are still bound to the speci ?c antigens Anti human IgG labelled with peroxidase is added The immuncomplex is visualized with TMB to give a blue reaction product Stop solution is added to stop the reaction and changing the colour of the reaction product into yellow Absorbance at nm is read using an ELISA microwell plate reader MATERIALS Reagents supplied Avidity Reagent bottle containing ml of an Urea solution coloured blue ready to use white cap Performance control bottle containing ml of a high avidity diluted serum coloured yellow ready to use green cap contains Bronidox L contains Kathon and Bronidox Additional materials required but not provided NovaTec CE-labelled Toxoplasma gondii IgG ELISA REF TOXG STABILITY AND STORAGE The reagents are stable up to the expiry date stated on the label when stored at C After ?rst opening the reagents are stable up to the expiry date REAGENT PREPARATION The avidity reagent and performance control are ready to use Crystallisation of the avidity reagent may occur at low temperature Therefore it is very important to bring the avidity reagent and control to room temperature ? C before starting the test run ASSAY PROCEDURE Use procedure and test preparation as mentioned in the NovaTec Toxoplasma gondii IgG package insert REF TOXG The performance of the avidity test is di ?erent as