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Guide rna synthesis Guide RNA Synthesis Gene Synthesis Blue Heron's patented GeneMaker technology provides the capability to synthesize any DNA sequences for you with few exceptions Please contact our tech support team with any questions OriGene shuttle v

Guide RNA Synthesis Gene Synthesis Blue Heron's patented GeneMaker technology provides the capability to synthesize any DNA sequences for you with few exceptions Please contact our tech support team with any questions OriGene shuttle vectors Express your insert in E coli or mammalian cells Low cost multiple functional vectors available Customer provided vectors Have your own vector Please submit your vector sequence and features through our secure website after registering Your vector is securely stored and can be used for future orders Blue Heron will require the physical vector once your order is placed CRISPR Cas Genome Editing The latest tool in genome editing ?? CRISPR Cas ?? allows for speci ?c genome disruption and replacement in a exible and simple system resulting in high speci ?city and low cell toxicity The CRISPR Cas genome editing system requires the co- expression of a Cas protein with a guide RNA vector expressed from the human U polymerase III promoter With the protospacer-adjacent motif PAM the sequence NGG present at the ?? end Cas will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA CExpression Vectors Blue Heron Bio is pleased to o ?er OriGene ? s a ?ordable PrecisionShuttle system for easy subcloning into a large selection of proven destination vectors for a variety of downstream applications Destination vector choices include ? Epitope tags MYC DDK etc for protein puri ?cation detection ? Fluorescent tags GFP RFP YFP etc for protein visualization ? Various drug markers neomycin puromycin etc for stable cell line establishment ? Inducible expression pTUNE ? E coli expression vectors ? Custom requests Expression Optimization Blue Heron Bio has developed a proprietary algorithm for protein expression optimization Based on codon usage and comprehensive knowledge of optimal RNA structure for protein translation Blue Heron can con ?dently deliver results in the desired organism Advantages Reliable ?? a proven algorithm successfully used by many researchers Survey Result Flexible ?? exclude unwanted restriction sites and special sequences use a customer ? s codon table add anking sequences Free ?? no charge for registered users Easy to use Procedure Login or create a new account Submit a protein sequence when starting with a DNA sequence please use our translation tool for conversion Select your model organism or your own codon table CCheck any restriction sites to be avoided optional Enter any special sequences to be avoided optional Add anking sequences optional An optimized sequence will be emailed and posted on your secure account Customer Example Protocol LGR and LGR proteins ? coding sequences were optimized using Blue Heron ? s proprietary algorithm Wild type and optimized sequences were cloned separately in pCMV-Entry vector Each DNA was transiently transfected to HEK T cells and incubated for hrs Proteins were puri ?ed using anti-DDK a ?nitive column and run on SDS PAGE then stained with Coomassie Blue For more information please visit http www blueheronbio com C